Studies on The Purification of Diphtheria Toxoid

The Purification and Concentration of Diphtheria Toxoid

By precipitation with acetone at 4 degrees C. a refined diphtheria toxoid was obtained as a dry powder, readily soluble in aqueous solutions. The powder itself appears to be stable. When dissolved in half the original volume of physiological salt solution, the toxoid remained stable, in the cold room, for a period of 7 months. Only toxoids should be used which have been completely detoxified by...

Study on Diphtheria Toxin Purification: A New Approach to Prepare Toxoid.

Comparative Studies on the Purification of Tetanus and Diphtheria Toxins.

Diphtheria toxin has been identified as a typical heat-coagulable protein (Eaton 1936 a, 1937; Pappenheimer 1937). If other toxins have chemical properties similar to diphtheria toxin, they might be isolated by similar methods. Using a gelatin hydrolysate medium, Pappenheimer and Johnson (1936, 1937) have produced diphtheria toxin which may be obtained in a relatively pure state by simple fract...

Study on Diphtheria Toxin Purification: A New Approach to Prepare Toxoid

Two produce a high potency diphtheria toxin two methods of detoxilïcation were applied. In the revised method the toxin was purified before detoxification by formaldehyde-stabilizing agent mixture. In the convention al method the crude toxin \Vas detoxified by formaldehyde alone. In this method culture lïItrates contain numerous impuritics and metabolites that may cause heterogenous product. Th...

Preparation and Evaluation of Diphtheria Toxoid-Containing Microspheres

Preparation of chitosan (CS) microspheres as a novel drug delivery vehicle for intranasal immunization using high, medium and low CS molecular weight (MW) was investigated in this study. Diphtheria toxoid (DT) was used as a model antigen. The emulsion-solidification method was adopted for microencapsulation of DT.  In the first step, following the purification of semi-crude DT by the ion-exchan...